業績

論文

• BRCA1 haploinsufficiency impairs iron metabolism to promote chrysotile-induced mesothelioma via ferroptosis resistance

  Yaguang Luo, Shinya Akatsuka, Yashiro Motooka, Yingyi Kong, Hao Zheng, Tomoji Mashimo, Tatsuhiko Imaoka, Shinya Toyokuni
  Cancer Science Volume 114, Issue 4, December 2022, 1423-1436

• Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner.

  Mori M, Dai J, Miyahara H, Li Y, Kang X, Yoshimi K, Mashimo T, Higuchi K, Matsumoto K.
  Exp Anim. 2022 Nov 29. doi: 10.1538/expanim.22-0122. PMID: 36450519

• Thioredoxin deficiency increases oxidative stress and causes bilateral symmetrical degeneration in rat midbrain

  Iori Ohmori, Mamoru Ouchida, Hirohiko Imai, Saeko Ishida, Shinya Toyokuni, Tomoji Mashimo
  Neurobiol Dis. Volume 175, December 2022, 105921

• A heterozygous LAMA5 variant may contribute to slowly progressive, vinculin-enhanced familial FSGS and pulmonary defects.

  Kaimori JY, Kikkawa Y, Motooka D, Namba-Hamano T, Takuwa A, Imai-Okazaki A, Kobayashi K, Tanigawa A, Kotani Y, Uno Y, Yoshimi K, Hattori K, Asahina Y, Kajimoto S, Doi Y, Oka T, Sakaguchi Y, Mashimo T, Sekiguchi K, Nakaya A, Nomizu M, Isaka Y.
  JCI Insight. 2022 Sep 29:e158378. doi: 10.1172/jci.insight.158378. PMID: 36173685

• The rat Downunder (Du) coat color mutation is associated with eye anomalies and embryonic lethality and maps to a 3.9-Mb region on chromosome 3.

  Hieu HT, Tanaka M, Kuwamura M, Mashimo T, Serikawa T, Kuramoto T.
  Exp Anim. 2022 Sep 19. doi: 10.1538/expanim.22-0086. PMID: 36123037

• Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the Combi-CRISPR technology.

  Matsushita N, Nishizawa K, Kato S, Iguchi Y, Fukabori R, Takeuchi K, Miyasaka Y, Mashimo T, Kobayashi K.
  J Neurosci Methods. 2022 Sep 9;381:109707. doi: 10.1016/j.jneumeth.2022.109707. PMID: 36089167

• Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3

  Kazuto Yoshimi, Kohei Takeshita, Noriyuki Kodera, Satomi Shibumura, Yuko Yamauchi, Mine Omatsu, Kenichi Umeda, Yayoi Kunihiro, Masaki Yamamoto & Tomoji Mashimo
  Nature Communications Volume 13, August 2022, 4917

• A high-quality severe combined immunodeficiency (SCID) rat bioresource

  Yoshiki Miyasaka,Jinxi Wang,Kosuke Hattori,Yuko Yamauchi,Miho Hoshi,Kazuto Yoshimi,Saeko Ishida,Tomoji Mashimo
  Plos one Volume 17, Issue 8, August 2022, e0272950

• Brca1L63X/+ rat is a novel model of human BRCA1 deficiency displaying susceptibility to radiation-induced mammary cancer

  Yuzuki Nakamura, Jo Kubota, Yukiko Nishimura, Kento Nagata, Mayumi Nishimura, Kazuhiro Daino, Atsuko Ishikawa, Takehito Kaneko, Tomoji Mashimo, Toshiaki Kokubo, Masaru Takabatake, Kazumasa Inoue, Masahiro Fukushi, Masami Arai, Mitsue Saito, Yoshiya Shimada, Shizuko Kakinuma, Tatsuhiko Imaoka
  Cancer Science Volume 113, Issue 10, July 2022, 3362-3375

• BRCA1 haploinsufficiency promotes chromosomal amplification under Fenton reaction-based carcinogenesis through ferroptosis-resistance

  Yingyi Kong, Shinya Akatsuka, Yashiro Motooka, Hao Zheng, Zhen Cheng, Yukihiro Shiraki, Tomoji Mashimo, Tatsuhiko Imaoka, Shinya Toyokuni
  Redox Biology Volume 54, August 2022, 102356

• Filaggrin-deficient rats generated using zinc-finger nucleases spontaneously exhibit dry scaly skin.

  Nakashima C, Doi H, Nakajima S, Mashimo T, Oga T, Ishida-Yamamoto A, Honda T, Ishida Y, Otsuka A, Kabashima K.
  Allergol Int. 2022 May 8:S1323-8930(22)00039-9. doi: 10.1016/j.alit.2022.04.001. PMID: 35545489

• Novel animal model of combined generalized and focal epilepsy

  Iori Ohmori, Mamoru Ouchida, Masakazu Shinohara, Kiyoka Kobayashi, Saeko Ishida, Tomoji Mashimo
  Epilepsia 2022 Jul;63(7):e80-e85. doi: 10.1111/epi.17295. Epub 2022 May 28. PMID: 35532890

• A humanised rat model of osteosarcoma reveals ultrastructural differences between bone and mineralised tumour tissue

  Christoph A. Lahr, Marietta Landgraf, Ferdinand Wagner, Amaia Cipitria, Inés Moreno-Jiménez, Onur Bas, Beat Schmutz, Christoph Meinert, Amanda Dos Santos Cavalcanti, Tomoji Mashimo, Yoshiki Miyasaka, Boris M. Holzapfel, Abbas Shafiee, Jacqui A. McGovern, Dietmar W. Hutmacher
  Bone Volume 158, 2022, 116018

• CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension

  Jane Chanda Kabwe, Hirofumi Sawada, Yoshihide Mitani, Hironori Oshita, Naoki Tsuboya, Erquan Zhang, Junko Maruyama, Yoshiki Miyasaka, Hideyoshi Ko, Kazunobu Oya, Hiromasa Ito, Noriko Yodoya, Shoichiro Otsuki, Hiroyuki Ohashi, Ryuji Okamoto, Kaoru Dohi, Yuhei Nishimura, Tomoji Mashimo, Masahiro Hirayama & Kazuo Maruyama
  Respiratory Research Volume 23, article number 87, April 2022

• CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus.

  Yoshimi K, Takeshita K, Yamayoshi S, Shibumura S, Yamauchi Y, Yamamoto M, Yotsuyanagi H, Kawaoka Y, Mashimo T.
  iScience. 2022 Jan 30;25(2):103830. doi: 10.1016/j.isci.2022.103830.

• Impact of GAD65 and/or GAD67 deficiency on perinatal development in rats.

  Jiang W, Kakizaki T, Fujihara K, Miyata S, Zhang Y, Suto T, Kato D, Saito S, Shibasaki K, Ishizaki Y, Isoda K, Yokoo H, Obinata H, Hirano T, Miyasaka Y, Mashimo T, Yanagawa Y.
  FASEB J. 2022 Feb;36(2):e22123. doi: 10.1096/fj.202101389R.

• Study of the CRISPR/Cas3 System for Xenotransplantation.

  Miyagawa S, Watanabe M, Nagashima H, Sato K, Kogata S, Toyama C, Masahata K, Kamiyama M, Yamamoto R, Eguchi H, Maeda A, Yoshimi K, Mashimo T, Okuyama H.
  Transplant Proc. 2022 Jan 11:S0041-1345(21)00905-2. doi: 10.1016/j.

• Dorsal telencephalon-specific Nprl2- and Nprl3-knockout mice: novel mouse models for GATORopathy.

  Ishida S, Zhao D, Sawada Y, Hiraoka Y, Mashimo T, Tanaka K.
  Hum Mol Genet. 2022 May 4;31(9):1519-1530. doi: 10.1093/hmg/ddab337. PMID: 34965576

• The role of cell-autonomous circadian oscillation of Cry transcription in circadian rhythm generation

  Ritsuko Matsumura, Kazuto Yoshimi, Yuka Sawai, Tsuyoshi Koide,, Koichi Node, Makoto Akashi
  Cell Reports Volume 39, Issue 3, 19 April 2022, 110703

著書・総説

• Genome editing technology and applications with the type I CRISPR system

  Kazuto Yoshimi, Tomoji Mashimo
  Gene and Genome Editing Volume 3-4, December 2022, 100013

• 遺伝子治療のためのゲノム編集技術

  藤井智明、真下知士
  メディカル・サイエンス・ダイジェスト2022年臨時増刊号、遺伝子治療の最前線 Vol.48, No.13, p6-9、ニュー・サイエンス社、2022年11月30日、ISBN1347-4340

• 「国産ゲノム編集ツールCRISPR-Cas3：遺伝子治療、診断への応用」再生医療

  吉見一人、真下知士
  日本再生医療学会雑誌 2022、Vol.21, p22-25

• 受精卵のゲノム編集

  石田紗恵子、真下知士
  HORMONE FRONTIER IN GYNECOLOGY Vol.29, No.1, p31-36、2022年3月1日、ISBN978-4-7792-2587-1

新聞・雑誌等

• 週刊エコノミスト「C4U国産のゲノム編集技術」国内未上場バイオベンチャー5社、2022年11月8日号
• 日経バイテク「東大医科研など、CRISPR-Cas3のメカニズムを解明」2022年9月8日
• 日本経済新聞オンライン「東大・理研・金沢大、国産ゲノム編集技術 CRISPR-Cas3が二本鎖DNAを切断する仕組みを解明」2022年8月29日
• NHK BSプレミアム ヒューマニエンス「“ゲノム編集” 新人類を生む技術か」2022年8月23日
• 共同通信2022年5月10日～「開発進む国産ゲノム編集　利用阻む特許の壁回避　精度と安全性向上目指す」
• ヘルシスト272 vol.46 No.2「細胞と遺伝子」第13回、河合香織「日本発！ゲノム編集技術CRISPR-Cas3」2022年3月10日発行第46巻第2号ISSN0389-6781
• 科学新聞2022年1月1日6面「先端的バイオ創薬等基盤技術開発事業」新規ゲノム編集技術を用いた次世代CAR-T細胞療法の開発