業績（2021年） | 東京大学医科学研究所実験動物研究施設先進動物ゲノム研究分野

論文

Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile.
Rika Hirano, Mikiyasu Sakanaka, Kazuto Yoshimi, Naohisa Sugimoto, Syogo Eguchi, Yuko Yamauchi, Misaki Nara, Shingo Maeda, Yuta Ami, Aina Gotoh, Takane Katayama, Noriho Iida, Tamotsu Kato, Hiroshi Ohno, Satoru Fukiya, Atsushi Yokota, Mamoru Nishimoto, Motomitsu Kitaoka, Hiroyuki Nakai, Shin Kurihara
Gut Microbes. Jan-Dec 2021;13(1):1973835. doi: 10.1080/19490976.2021.1973835.
Dorsal telencephalon-specific Nprl2- and Nprl3-knockout mice: novel mouse models for GATORopathy.
Ishida S, Zhao D, Sawada Y, Hiraoka Y, Mashimo T, Tanaka K.
Hum Mol Genet. 2021 Nov 20:ddab337. doi: 10.1093/hmg/ddab337.
Aberrant accumulation of TMEM43 accompanied by perturbed transmural gene expression in arrhythmogenic cardiomyopathy.
Shinomiya H, Kato H, Kuramoto Y, Watanabe N, Tsuruda T, Arimura T, Miyashita Y, Miyasaka Y, Mashimo T, Takuwa A, Motooka D, Okuzaki D, Matsuoka K, Tsukamoto O, Hakui H, Yamada N, Lee JK, Kioka H, Kitakaze M, Takashima S, Sakata Y, Asano Y.
FASEB J. 2021 Nov;35(11):e21994. doi: 10.1096/fj.202100800R.
A humanised rat model reveals ultrastructural differences between bone and mineralised tumour tissue.
Lahr CA, Landgraf M, Wagner F, Cipitria A, Moreno-Jiménez I, Bas O, Schmutz B, Meinert C, Mashimo T, Miyasaka Y, Holzapfel BM, Shafiee A, McGovern JA, Hutmacher DW.
Bone. 2021 May 20:116018. doi: 10.1016/j.bone.2021.116018.
Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation".
Gurumurthy CB, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb K, Eudy JD, Gan L, Gao J, Gonzales A, Guntur A, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majeweska L, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford A, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G.
Genome Biol. 2021 Apr 7;22(1):99. doi: 10.1186/s13059-021-02320-3.
Deletion of B-cell translocation gene 2 (BTG2) alters the responses of glial cells in white matter to chronic cerebral hypoperfusion.
Suzuki K, Shinohara M, Uno Y, Tashiro Y, Gheni G, Yamamoto M, Fukumori A, Shindo A, Mashimo T, Tomimoto H, Sato N.
J Neuroinflammation. 2021 Apr 3;18(1):86. doi: 10.1186/s12974-021-02135-w.
Behavioral Consequences of a Combination of Gad1 Haplodeficiency and Adolescent Exposure to an NMDA Receptor Antagonist in Long-Evans Rats.
Fujihara K, Sato T, Higeta K, Miyasaka Y, Mashimo T, Yanagawa Y.
Front Pharmacol. 2021 Mar 30;12:646088. doi: 10.3389/fphar.2021.646088.
PHF24 is expressed in the inhibitory interneurons in rats.
Numakura Y, Uemura R, Tanaka M, Izawa T, Yamate J, Kuramoto T, Kaneko T, Mashimo T, Yamamoto T, Serikawa T, Kuwamura M.
Exp Anim. 2021 Feb 6;70(1):137-143. doi: 10.1538/expanim.20-0105.
Genetic deletion of the 67-kDa isoform of glutamate decarboxylase alters conditioned fear behavior in rats.
Fujihara K, Sato T, Miyasaka Y, Mashimo T, Yanagawa Y.
FEBS Open Bio. 2021 Feb;11(2):340-353. doi: 10.1002/2211-5463.13065.
Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats.
Yoshimi K, Oka Y, Miyasaka Y, Kotani Y, Yasumura M, Uno Y, Hattori K, Tanigawa A, Sato M, Oya M, Nakamura K, Matsushita N, Kobayashi K, Mashimo T.
Hum Genet. 2021 Feb;140(2):277-287. doi: 10.1007/s00439-020-02198-4.
Pathophysiological significance of Stim1 mutation in sympathetic response to stress and cardiovascular phenotypes in SHRSP/Izm: In vivo evaluation by creation of a novel gene knock-in rat using CRISPR/Cas9.
Odongoo B, Ohara H, Ngarashi D, Kaneko T, Kunihiro Y, Mashimo T, Nabika T.
Clin Exp Hypertens. 2021 Jan 2;43(1):34-41. doi: 10.1080/10641963.2020.1797085.

著書・総説

国産ゲノム編集ツールCRISPR-Cas3の遺伝子治療への応用

小児科診療 UP-to-DATE Vol.51、p20-24、2021年8月24日
新型コロナウイルスとゲノム編集
真下知士
フナコシニュース「ゲノム編集 2021」 2021年6月15日号（No.728）
CRISPR技術を用いた核酸検出
吉見一人、真下知士
実験医学「CRISPR最新ツールボックス」 Vol.39、No.8、P1214-18、2021年5月1日
効率的なゲノム編集ラットの作製方法
服部晃佑、吉見一人、松下夏樹、小林和人、真下知士
マウス・ラットモデル作製・解析プロフェッショナル p54-63、2021年03月18日
国産ゲノム編集技術CRISPR-Cas3の特徴とCRISPR診断への応用
吉見一人、真下知士
最新のゲノム編集技術と用途展開 P51-59、2021年2月8日

業績 2021

論文

Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile.

Dorsal telencephalon-specific Nprl2- and Nprl3-knockout mice: novel mouse models for GATORopathy.

Aberrant accumulation of TMEM43 accompanied by perturbed transmural gene expression in arrhythmogenic cardiomyopathy.

A humanised rat model reveals ultrastructural differences between bone and mineralised tumour tissue.

Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation".

Deletion of B-cell translocation gene 2 (BTG2) alters the responses of glial cells in white matter to chronic cerebral hypoperfusion.

Behavioral Consequences of a Combination of Gad1 Haplodeficiency and Adolescent Exposure to an NMDA Receptor Antagonist in Long-Evans Rats.

PHF24 is expressed in the inhibitory interneurons in rats.

Genetic deletion of the 67-kDa isoform of glutamate decarboxylase alters conditioned fear behavior in rats.

Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats.

Pathophysiological significance of Stim1 mutation in sympathetic response to stress and cardiovascular phenotypes in SHRSP/Izm: In vivo evaluation by creation of a novel gene knock-in rat using CRISPR/Cas9.

著書・総説

国産ゲノム編集ツールCRISPR-Cas3の遺伝子治療への応用

新型コロナウイルスとゲノム編集

CRISPR技術を用いた核酸検出

効率的なゲノム編集ラットの作製方法

国産ゲノム編集技術CRISPR-Cas3の特徴とCRISPR診断への応用