Metabolic plasticity of hematopoietic stem cells
"Metabolic plasticity of hematopoietic stem cells"
Seminar Date and Time: 2/12/2020 (Wed) 15:05 ~ 15:35
Venue: Building 1, 2-3 conference room
Speaker (Name): Keiyo Takubo
Project Leader, Department of Stem Cell Biology, National Center for Global Health and Medicine Research Institute, Tokyo
Subject: Metabolic plasticity of hematopoietic stem cells
Metabolic dynamics control the fate of hematopoietic stem and progenitor cells (HSPCs), whether they proliferate, differentiate, or keep quiescence in cell cycle. Despite its importance, the metabolic difference between the most primitive hematopoietic stem cells (HSCs) and progenitors is yet unclear due to paucity of methods that can quantitatively measure metabolic profile. For instance, to what extent each of the HSPC fraction depends on glycolysis and mitochondrial oxidative phosphorylation is still an open question. Adenosine triphosphate (ATP) plays a central role in metabolism and kinetics of which represents metabolic status inside cells. We took advantage of ATP biosensor GO-ATeam2-knock-in mice to quantify ATP concentration within the HSPC fraction with high temporal resolution at single cell level thereby examining how HSPCs adapt to nutrient environment, cell-cycle status and metabolic perturbation. In this seminar, I will present our approaches to clarify metabolic programs including "metabolic plasticity" of HSCs which is unique among HSPCs.
Organizer (Host Researcher): Atsushi Iwama (Stem Cell and Molecular Medicine)