A new method to visualize cerebral malaria
At November 2021 issue of International Immunology, Volume 33, Issue 11, November 2021, Pages 587–594, https://doi.org/10.1093/intimm/dxab060.
Cerebral malaria (CM) is the major cause of death in Plasmodium falciparum-infected humans. The mechanisms are not fully established although sequestration of infected erythrocytes in microvessels is thought to play an important role. In their Short Communication,Matsuo-Dapaah et al.construct 3-dimensionl images of single microvessels deep in the whole brain using a new method—clear, unobstructed, brain/body imaging cocktails and computational analysis (CUBIC) with light sheet fluorescent microscopy (LSFM). They use this method in experimental cerebral malaria, in which mice are infected with Plasmodium berghei ANKA. The parasites are shown to preferentially accumulate in the capillaries and tissue of the olfactory bulb (see figure) rather than other areas of the brain. If the bulk of the olfactory bulb is removed, parasites are shown to accumulate in the brainstem. This method should prove valuable for efforts to characterize the interactions between infected erythrocytes and blood microvessels in the brain and may even be applicable to post-mortem human tissue.