Selected articles

Yoshioka D, Nakamura T, Kubota Y, and Takekawa M.
Formation of the NLRP3 inflammasome inhibits stress granule assembly by multiple mechanisms.
J. Biochem. mvae009, https://doi.org/10.1093/jb/mvae009 (2024)

Fujikawa D, Nakamura T, Yoshioka D, Li Z, Moriizumi H, Taguchi M, Tokai-Nishizumi N, Kozuka-Hata H, Oyama M, and Takekawa M.
Stress granule formation inhibits stress-induced apoptosis by selectively sequestering executioner caspases.
Current Biology 33: 1967-1981.e8. doi: 10.1016/j.cub.2023.04.012 (2023)

Amada K, Hijiya N, Ikarimoto S, Yanagihara K, Hanada T, Hidano S, Kurogi S, Tsukamoto Y, Nakada C, Kinoshita K, Hirashita Y, Uchida T, Shin T, Yada K, Hirashita T, Kobayashi T, Murakami K, Inomata M, Shirao K, Aoki M, Takekawa M, Moriyama M.
Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor.
Cancer Science. doi.org/10.1111/cas.15735 (2023)

Moriizumi H, Kubota Y, Tsuchiya T, Naka R, and Takekawa M.
Caspase 3-specific cleavage of MEK1 suppresses ERK signaling and sensitizes cells to stress-induced apoptosis.
FEBS Open Bio. doi.org/10.1002/2211-5463.13574 (2023)

Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, and Takekawa M.
ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.
Nature Commun. 13:7476. doi: 10.1038/s41467-022-35230-4 (2022)

Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda N.N., and Takekawa M.
Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.
Nature Commun. 13: 4063 doi: 10.1038/s41467-022-31690-w (2022)

Tsukamoto Y, Kurogi S, Shibata T, Suzuki K, Hirashita Y, Fumoto S, Yano S, Yanagihara K, Nakada C, Mieno F, Kinoshita K, Fuchino T, Mizukami K, Ueda Y, Etoh T, Uchida T, Hanada T, Takekawa M, Daa T, Shirano K, Hironaka S, Murakami K, Inomata M, Hijiya N, and Moriyama M.
Enhanced phosphorylation of c-Jun by cisplatin treatment as a potential predictive biomarker for cisplatin response in combination with patient-derived tumor organoids.
Lab Invest. doi:10.1038/s41374-022-00827-2 (2022)

Watanabe M, Arii J, Takeshima K, Fukui A, Shimojima M, Kozuka-Hata H, Oyama M, Minamitani T, Yasui T, Kubota Y, Takekawa M, Kosugi I, Maruzuru Y, Koyanagi N, Kato A, Mori Y, and Kawaguchi Y.
Prohibitin-1 contributes to the cell-to-cell transmission of herpes simplex virus 1 via the MAPK/ERK signaling pathway.
Journal of Virology 95: e01413-20 doi: 10.1128/JVI.01413-20 (2021)

Matsushita M, Nakamura T, Moriizumi H, Miki H, and Takekawa M.
Stress-responsive MTK1 SAPKKK serves as a redox sensor that mediates delayed and sustained activation of SAPKs by oxidative stress.
Science Advances 6:eaay9778 doi:10.1126/sciadv.aay9778 (2020)　【 Link 】

Weng JS, Nakamura T, Moriizumi H, Takano H, Yao R, and Takekawa M.
MCRIP1 promotes the expression of lung-surfactant proteins in mice by disrupting CtBP-mediated epigenetic gene silencing.
Commun Biol. 2:227 doi:10.1038/s42003-019-0478-3 (2019)

Fujioka K, Kubota Y, and Takekawa M.
A novel method for the detection of O-GlcNAc-modified proteins by Lectin affinity gel electrophoresis.
Bio-protocol. 8: e3098, doi:10.21769/BioProtoc.3098 (2018)

Ichimanda M, Hijiya N, Tsukamoto Y, Uchida T, Nakada, C, Akagi T, Etoh T, Iha H, Inomata M, Takekawa M and Moriyama M.
Downregulation of DUSP4 enhances cell proliferation and invasiveness in colorectal carcinomas.
Cancer Sci. 109: 250-258 (2018)

Kubota Y, Fujioka K and Takekawa M.
WGA-based lectin affinity gel electrophoresis: a novel method for the detection of O-GlcNAc-modified proteins.
PLoS ONE 12: e0180714. doi:10.1371/journal.pone.0180714 (2017)

Iijima M, Kubota Y, Sawa R, Kubota Y, Hatano M, Igarashi M, Kawada M, Momose I, Takekawa M and Shibasaki M.
A guanine derivative as a new MEK inhibitor produced by Streptomuces sp. MK63-43F2.
J Antibiot. 71: 135-138 (2017)

Arimoto-Matsuzaki K, Saito H and Takekawa M.
TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis.
Nature Commun. 7: 10252 doi:10.1038/ncomms10252 (2016)

Hijiya N, Tsukamoto Y, Nakada C, Tung NL, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, and Moriyama M.
Genomic loss of DUSP4 contributes to the progression of intraepitherial neoplasm of pancreas to invasive carcinoma.
Cancer Res. 76: 2612-2625 (2016)

Kinoshita E, Kinoshita-Kikuta E, Kubota Y, Takekawa M and Koike T.
A Phos-tag SDS-PAGE method that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1.
Proteomics 16: 1825-1836 (2016)

Kubota Y and Takekawa M.
Detection and functional analysis of SUMO-modified MEK.
Methods in Molecular Biology, Springer Press, in press (2016)

Ichikawa K, Kubota Y, Nakamura T, Weng JS, Tomida T, Saito H and Takekawa M.
MCRIP1, an ERK substrate, mediates ERK-induced gene silencing during epithelial-mesenchymal transition by regulating the co-repressor CtBP.
Molecular Cell 58: 35-46 (2015) [Abstract] [Faculty of 1000]

Tomida T, Takekawa M and Saito H.
Oscillation of p38 activity controls efficient pro-inflammatory gene expression.
Nature Commun. 6: 8350 doi:10.1038/ncomms9350 (2015)

Ohshima D, Arimoto-Matsuzaki K, Tomida T, Takekawa M and Ichikawa K.
Spatio-temporal dynamics and mechanisms of stress-granule assembly.
PLoS Comput. Biol. 11: e1004326 doi:10.1371/journal.pcbi.1004326 (2015)

Takekawa M and Kubota Y.
Mitogen-activated protein kinase signaling and cancer.
Protein Modifications in Pathogenic Dysregulation of Signaling, Springer Press, 211-231 (2015)

Ohshima D, Arimoto-Matsuzaki K, Tomida T, Takekawa M and Ichikawa K.
Stochastic Simulation of Stress Granules.
Protein Modifications in Pathogenic Dysregulation of Signaling, Springer Press, 77-93 (2015)

Sato Y, Goto E, Shibata Y, Kubota Y, Yamagata A, Goto-Ito S, Kubota K, Inoue J, Takekawa M, Tokunaga F,
Fukai S.
Structures of CYLD USP with Met1- or Lys63-linked diubiquitin reveal mechanisms for dual specificity.
Nature Struct. & Mol. Biol. 22: 222-229 (2014)

Nakamura T, Saito H and Takekawa M.
SAPK pathways and p53 cooperatively regulate PLK4 activity and centrosome integrity under stress.
Nature Commun. 4:1775 doi:10.1038/ncomms2752 (2013)

Tomida T, Osa S, Takekawa M, Iino Y and Saito H.
The temporal pattern of stimulation determines the extent and duration of MAPK activation in a caenorhabditis elegans sensory neuron.
Science Signaling 5, ra76 (2012)

Kubota Y, O’Grady P, Saito H and Takekawa M.
Oncogenic Ras abrogates MEK SUMOylation that suppresses the ERK pathway and cell transformation.
Nature Cell Biol. 13, 282-291 (2011) [Abstract] [Faculty of 1000]
【 Link 】： “ Editor’s Choice ” in Science Signaling / “ Highlights ” in A-IMBN Research etc.

Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, and Koike K.
Receptor for activated protein kinase C: Requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma.
PLoS ONE 6, e24359 (2011)

Takekawa M, Kubota Y, Nakamura T and Ichikawa K.
Regulation of stress-activated MAP kinase pathways during cell fate decisions. (review)
Nagoya J. Med. Sci. 73, 1-14 (2010)

Tomida T, Takekawa M, O’Grady P and Saito H.
Stimulus-specific distinctions in spatial and temporal dynamics of stress-activated protein kinase kinase kinases revealed by FRET biosensor.
Mol. Cell. Biol. 29, 6117-6127 (2009)

Arimoto K, Fukuda H, Imajoh-Ohmi S, Saito H, and Takekawa M.
Formation of stress granules inhibits apoptosis by suppressing stress-responsive MAPK pathways.
Nature Cell Biol. 10, 1324-1332 (2008)
＊紹介記事【 Link 】： “ Editor’s Choice ” in Science / Science Signaling / Nature Signaling Gateway

Miyake Z, Takekawa M, Ge Q and Saito H.
Activation of MTK1/MEKK4 by GADD45 through induced N-C dissociation and dimerization-mediated trans-autophosphorylation of the MTK1 kinase domain.
Mol. Cell. Biol. 27, 2765-2776 (2007)

Nakajima A, Komazawa-Sakon S, Takekawa M, Sasazuki T, Yeh W-C, Yagita H, Okumura K and Nakano H.
An antiapoptotic protein, c-FLIPL, directly binds to MKK7 and inhibits the JNK pathway.
EMBO J. 25, 5549–5559 (2006)

Fujimoto H, Onishi N, Kato N, Takekawa M, Xu X-Z, Kosugi A, Kondo T, Imamura M, Yoda A, Oishi I, and Minami Y.
Regulation of the anti-oncogenic Chk2 kinase by the oncogenic Wip1 phosphatase.
Cell Death Differ. 13, 1170-1180 (2006)

Takekawa, M., Tatebayashi, K., and Saito, H.
Conserved docking site is essential for activation of mammalian MAP kinase kinases by specific MAP kinase kinase kinases.
Molecular Cell 18, 295-306 (2005)

Chi H, Lu B, Takekawa M, Davis RJ, and Flavell RA.
GADD45β/GADD45γ and MEKK4 comprise a genetic pathway mediating STAT-independent IFNγ production in T cells.
EMBO J. 23, 1576–1586 (2004)

Takagaki K, Satoh T, Tanuma N, Masuda K, Takekawa M, Shima H and Kikuchi K.
Characterization of a novel low-molecular-mass dual-specificity phosphatase 3 (LDP-3) that enhances activation of JNK and p38.
Biochemical J. 383, 447-455 (2004)

Sakon S, Xue X, Takekawa M, Sasazuki T, Okazaki T, Kojima Y, Pia J-H, Yagita H, Okumura K, Doi T and Nakano H.
NF-κB inhibits TNF-induced accumulation of ROS that mediate prolonged MAPK activation and necrotic cell death.
EMBO J. 22, 3898-3909 (2003)

Tatebayashi K, Takekawa M and Saito H.
A docking site determining specificity of Pbs2 MAPKK to Ssk2/Ssk22 MAPKKKs in yeast HOG pathway.
EMBO J. 22, 3624-3634, (2003)

Mitsuhashi S, Shima H, Tanuma N, Matsuura N, Takekawa M, Urano T, Kataoka T, Ubukata M and Kikuchi K. Usage of tautomycetin, a novel inhibitor of protein phosphatase 1 (PP1) reveals that PP1 is a positive regulator of Raf-1 in COS-7 cells.
J. Biol. Chem. 278, 82-88, (2003)

Takekawa M, Tatebayashi K, Itoh F, Adachi M, Imai K and Saito H.
Smad-dependent GADD45β expression mediates delayed activation of p38 MAP kinase by TGF-β.
EMBO J. 21, 6473-6482 (2002) 【 Full-scan画像 】

Mita H, Tsutsui J, Takekawa M, Witten EA and Saito H.
Regulation of MTK1/MEKK4 kinase activity by its N-terminal autoinhibitory domain and GADD45 binding.
Mol. Cell. Biol. 22, 4544-4555, (2002)

Shonai T, Adachi M, Sakata K, Takekawa M, Endo T, Imai K and Hareyama M.
MEK/ERK pathway protects ionizing radiation-induced loss of mitochondrial membrane potential and cell death in lymphocytic leukemia cells.
Cell Death Differ. 9, 963-971, (2002)

Itoh M, Adachi M, Yasui H, Takekawa M, Tanaka H and Imai K.
Nuclear export of glucocorticoid receptor is enhanced by JNK-mediated phosphorylation.
Mol. Endocrinol. 16, 2382-2392, (2002)

Matsunaga T, Ishida T, Takekawa M, Nishimura S, Adachi M and Imai K.
Analysis of gene expression during maturation of immature dendritic cells derived from peripheral blood monocytes.
Scand. J. Immunol. 56, 593-601, (2002)

Nakamura K, Tanoue K, Satoh T, Takekawa M, Watanabe M, Shima H and Kikuchi K.
A novel low-molecular-mass dual-specificity phosphatase, LDP-2, with a naturally occurred substitution that affects substrate specificity.
J. Biochem. 132, 463-470 (2002)

Takekawa M, Adachi M, Nakahata A, Nakayama I, Itoh F, Tsukuda H, Taya Y and Imai K.
p53-inducible Wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV radiation.
EMBO J., 19, 6517-6526, (2000)

Koyama Y, Adachi M, Sekiya M, Takekawa M and Imai K.
Histone deacetylase inhibitors suppress IL-2-mediated gene expression prior to induction of apoptosis.
Blood 96, 1490-1495, (2000)

Kaneki M, Kharbanda S, Pandey P, Yoshida K, Takekawa M, Liou J-R, Stone R and Kufe D.
Functional role for protein kinase Cβ as a regulator of stress-activated protein kinase activation and monocytic differentiation of myeloid leukemia cells.
Mol. Cell. Biol. 19, 461-470, (1999)

Takekawa M and Saito H.
A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK.
Cell 95, 521-530, (1998)

Takekawa M, Maeda T and Saito H.
Protein phosphatase 2Cα inhibits the human stress-responsive p38 and JNK MAPK pathways.
EMBO J. 17, 4744-4752, (1998)

Posas F, Takekawa M and Saito H.
Signal transduction by MAP kinase cascades in budding yeast.
Curr. Opin. Microbiol. 1, 175-182, (1998)

Takekawa M, Posas F and Saito H.
A human homolog of the yeast Ssk2/Ssk22 MAP kinase kinase kinase, MTK1, mediates stress-induced activation of the p38 and JNK pathways.
EMBO J. 16, 4973-4982, (1997)

Maeda T, Takekawa M and Saito H.
Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor.
Science 269, 554-558, (1995)

Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, Imai K and Yachi A.
Chromosomal localization of protein tyrosine phosphatase G1 gene and characterization of aberrant transcripts in human colon cancer cells.
FEBS Lett. 339, 222-228, (1994)

Adachi M, Maeda K, Takekawa M, Hinoda Y, Imai K, Sugiyama S and Yachi A.
High expression of CD56 (N-CAM) in a patient with cutaneous CD4-positive lymphoma.
Am. J. Hematol. 47, 278-282, (1994)

Adachi M, Sekiya M, Arimura Y, Takekawa M, Itoh F, Hinoda Y, Imai K and Yachi A.
Protein-tyrosine phosphatase expression in pre-B cell NALM-6.
Cancer Res. 52, 737-740, (1992)

Takekawa M, Itoh F, Hinoda Y, Arimura Y, Toyota M, Sekiya M, Adachi M, Imai K and Yachi A.
Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein tyrosine phosphatase.
Biochem. Biophys. Res. Commun. 189, 1223-1230, (1992)

Arimura Y, Hinoda Y, Itoh F, Takekawa M, Tsujisaki M, Adachi M, Imai K and Yachi A.
cDNA cloning of new protein-tyrosine-phosphatases in human colon.
Tumor Biol. 13, 180-186, (1992)

Takekawa M, Imai K, Adachi M, Aoki S, Maeda K, Hinoda Y and Yachi A.
Hypereosinophilic syndrome accompanied with necrosis of finger tips.
Internal Med. 31, 1262-1266, (1992)