"Discovery of nucleocytosis reveals immune-activation via the extraction of nuclear DNA"
Nature Communications Online 18 February 2026 doi:10.1038/s41467-026-68839-w
Novel Cellular Phenomenon Reveals How Immune Cells Extract Nuclear DNA from Dying Cells
Description
Published Article
Hideo Negishi1,2,3,4,#, Yusuke Wada1,#, Yoshitaka Shirasaki5,#, Tomoya Hayashi1,2,4, Yuji Kubota6, Tomio Iwasaki7, Mina Kurosawa1, Tatsuma Ban8, Daisuke Muto9,26, Yusuke Suenaga9, Taichi Kojima1, Yuzuki Matsuda1, Sean Lord Irish1, Kosuke Dodo10,11, Toru Suzuki12, Mai Yamagishi13, Burcu Temizoz1,2,4, Atsushi Yoshimori14, Chisato Kanai15, Yoji Nagasaki16, Masaki Ohmuraya17, Tomohiko Tamura8,18, Atsushi Iwama4,19,20, Toshifumi Inada4,12, Etsushi Kuroda21, Kouji Kobiyama1,2,4,22, Noriko Toyama-Sorimachi2, Mutsuhiro Takekawa4,6,23, Cevayir Coban2,4,24, and Ken J. Ishii1,2,4,25
1Division of Vaccine Science, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
2International Vaccine Design Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
3Laboratory of Immunology and Infection Control, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan
4The University of Tokyo Pandemic Preparedness, Infection and Advanced Research Center (UTOPIA), The University of Tokyo, Tokyo, Japan
5Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan
6Division of Cell Signaling and Molecular Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
7Research and Development Group, Hitachi, Ltd., Ibaraki, Japan
8Department of Immunology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan
9Laboratory of Evolutionary Oncology, Chiba Cancer Center Research Institute, Chiba, Japan
10Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, Saitama, Japan
11Catalysis and Integrated Research Group, RIKEN Center for Sustainable Resource Science, Saitama, Japan
12Division of RNA and Gene Regulation, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
13Live Cell Diagnosis, Ltd., Saitama, Japan
14Institute for Theoretical Medicine, Inc., Kanagawa, Japan
15INTAGE Healthcare, Inc., Osaka, Japan
16Department of Pharmacy, NHO Kyushu Medical Center, Fukuoka, Japan
17Department of Genetics, School of Medicine, Hyogo Medical University, Hyogo, Japan
18Advanced Medical Research Center, Yokohama City University, Kanagawa, Japan
19Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
20Laboratory of Cellular and Molecular Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
21Department of Immunology, Hyogo Medical University School of Medicine, Hyogo, Japan
22Current address: Division of Rheumatology, Department of Medicine, University of California, San Diego, CA, USA
23Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
24Division of Malaria Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
25Laboratory of Vaccine Science, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
26School of Medical and Pharmaceutical Sciences, Chiba University, Chiba, Japan
#These authors contributed equally: Hideo Negishi, Yusuke Wada, and Yoshitaka Shirasaki
1Division of Vaccine Science, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
2International Vaccine Design Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
3Laboratory of Immunology and Infection Control, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan
4The University of Tokyo Pandemic Preparedness, Infection and Advanced Research Center (UTOPIA), The University of Tokyo, Tokyo, Japan
5Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan
6Division of Cell Signaling and Molecular Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
7Research and Development Group, Hitachi, Ltd., Ibaraki, Japan
8Department of Immunology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan
9Laboratory of Evolutionary Oncology, Chiba Cancer Center Research Institute, Chiba, Japan
10Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, Saitama, Japan
11Catalysis and Integrated Research Group, RIKEN Center for Sustainable Resource Science, Saitama, Japan
12Division of RNA and Gene Regulation, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
13Live Cell Diagnosis, Ltd., Saitama, Japan
14Institute for Theoretical Medicine, Inc., Kanagawa, Japan
15INTAGE Healthcare, Inc., Osaka, Japan
16Department of Pharmacy, NHO Kyushu Medical Center, Fukuoka, Japan
17Department of Genetics, School of Medicine, Hyogo Medical University, Hyogo, Japan
18Advanced Medical Research Center, Yokohama City University, Kanagawa, Japan
19Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
20Laboratory of Cellular and Molecular Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
21Department of Immunology, Hyogo Medical University School of Medicine, Hyogo, Japan
22Current address: Division of Rheumatology, Department of Medicine, University of California, San Diego, CA, USA
23Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
24Division of Malaria Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
25Laboratory of Vaccine Science, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
26School of Medical and Pharmaceutical Sciences, Chiba University, Chiba, Japan
#These authors contributed equally: Hideo Negishi, Yusuke Wada, and Yoshitaka Shirasaki
