"A scalable two-step genome editing strategy for generating full-length gene-humanized mice at diverse genomic loci"
Nature Communications Online 14 January 2026 doi:10.1038/s41467-025-67900-4
Two-Step Genome Editing Enables the Creation of Full-Length Humanized Mouse Models
Description
Published Article
Jumpei Taguchi1, Mio Kikuchi1, Hyojung Jeon2, Ryo Shimizu3, Hideto Mori4, Masahito Ikawa5,6, Yasuhiro Yamada7, Kei Sato8, Terumasa Ikeda3, Satoshi Yamazaki1,2, and Manabu Ozawa1,6
1Core Laboratory for Developing Advanced Animal Models, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Japan
2Division of Cell Regulation, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Japan
3Division of Molecular Virology and Genetics, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Japan
4Premium Research Institute for Human Metaverse Medicine (WPI-PRIMe), The University of Osaka, Japan
5Department of Experimental Genome Research, Research Institute for Microbial Diseases, The University of Osaka, Japan
6Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Japan
7Department of Molecular Pathology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Japan
8Division of Systems Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Japan
1Core Laboratory for Developing Advanced Animal Models, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Japan
2Division of Cell Regulation, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Japan
3Division of Molecular Virology and Genetics, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Japan
4Premium Research Institute for Human Metaverse Medicine (WPI-PRIMe), The University of Osaka, Japan
5Department of Experimental Genome Research, Research Institute for Microbial Diseases, The University of Osaka, Japan
6Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Japan
7Department of Molecular Pathology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Japan
8Division of Systems Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Japan
