Viral dark matter exposed: metagenome database detects phage-derived antibacterial enzyme
Viral dark matter exposed: metagenome database detects phage-derived antibacterial enzyme.
Cell Host & Microbe on July 11, 2020. DOI：10.1016/j.chom.2020.06.005
In a pioneer study published in Cell Host & Microbe – Researchers at Osaka City University and The Institute of Medical Science, The University of Tokyo, reported intestinal bacterial and viral metagenome information from the fecal samples of 101 healthy Japanese individuals. This analysis, leveraging host bacteria–phage associations, detected phage-derived antibacterial enzymes that control pathobionts. As proof-of-concept, phage-derived endolysins are shown to regulate C. difficile infection in mice.
1Department of Immunology and Genomics, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan
2Division of Metagenome Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
3Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
4Division of Systems Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
5Mucosal Vaccine Project, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan
6Laboratory of DNA Information Analysis, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
7Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
8Division of Clinical Genome Research, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
9Department of Computer Science, Tokyo Institute of Technology, Tokyo 152-8550, Japan
10Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
11Division of Gastroenterology, Department of Medicine, CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines, University of California San Diego, La Jolla, CA 92093, USA
12Division of Comparative Pathology and Medicine, Department of Pathology, University of California San Diego, La Jolla, CA 92093, USA
13Center for Veterinary Sciences and Comparative Medicine, University of California San Diego, La Jolla, CA 92093, USA
14Department of Mucosal Immunology, IMSUT Distinguished Professor Unit, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
15International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
16Division of Health Medical Data Science, Health Intelligence Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
17Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo 113-8657, Japan
18Lead contact
1Department of Immunology and Genomics, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan
2Division of Metagenome Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
3Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
4Division of Systems Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
5Mucosal Vaccine Project, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan
6Laboratory of DNA Information Analysis, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
7Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
8Division of Clinical Genome Research, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
9Department of Computer Science, Tokyo Institute of Technology, Tokyo 152-8550, Japan
10Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
11Division of Gastroenterology, Department of Medicine, CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines, University of California San Diego, La Jolla, CA 92093, USA
12Division of Comparative Pathology and Medicine, Department of Pathology, University of California San Diego, La Jolla, CA 92093, USA
13Center for Veterinary Sciences and Comparative Medicine, University of California San Diego, La Jolla, CA 92093, USA
14Department of Mucosal Immunology, IMSUT Distinguished Professor Unit, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
15International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
16Division of Health Medical Data Science, Health Intelligence Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
17Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo 113-8657, Japan
18Lead contact
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