Establish a New Frontier of Stem Cell Therapy by Connecting the Basic Science and Clinical Medicine.

We are working to uncover new diseases, elucidating the causes of disease and developing therapeutic modalities by connecting the knowledge and methodology of basic science such as immunology, molecular biology, cell biology and developmental engineering with clinical medicine.

Current NewsList

2021/11/26

Dr.Yanagida Selected proposals for FY2021 call (in Japanese only) has been decided

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2021/09/30

Article on Nature

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2021/04/24

Dr.Kano was awarded the JES Young Investigator

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2020/12/29

PRESS:Generation of Functional Organs Using a Cell Competitive Niche in Intra-and Inter-species Rodent Chimeras.

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2019/11/13

PRESS:Generation of functional lungs via conditional blastocyst complementation using pluripotent stem cells.

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2019/09/03

Participated of AMED再生医療研究交流会

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2019/08/01

AFP Article

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2019/07/30

Article on INDEPENDENT

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2019/07/26

Article on Nature

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2019/05/30

"Long term ex vivo hematopoietic stem cell expansion allows nonconditioned transplantation" was published in "Nature".

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PublicationsList

Yamazaki K, Kubara K, Ishii S, Li P, Dairiki R, Hihara T, Ishizuka Y, Izumi Y, Kumai M, Kamisako T, Ishizaki H, Sato H, Masaki H, Mizuno N, Mitsuhashi K, Ito M, Hamanaka S, Yamaguchi T, Watanabe M, Sugiyama F, Nakauchi H.
In vitro and in vivo functions of T cells produced in complemented thymi of chimeric mice generated by blastocyst complementation.
Sci Rep. 2022 Feb

Abstract on PubMed

Toral MA, Charlesworth CT, Ng B, Chemudupati T, Homma S, Nakauchi H, Bassuk AG, Porteus MH, Mahajan VB.
Investigation of Cas9 antibodies in the human eye.
Nat Commun. 2022 Feb

Abstract on PubMed

Oikawa M, Nagae M, Mizuno N, Iwatsuki K, Yoshida F, Inoue N, Uenoyama Y, Tsukamura H, Nakauchi H, Hirabayashi M, Kobayashi T.
Generation of Tfap2c-T2A-tdTomato knock-in reporter rats via adeno-associated virus-mediated efficient gene targeting.
Mol Reprod Dev. 2022 Feb

Abstract on PubMed

Harada S, Ando M, Ando J, Ishii M, Yamaguchi T, Yamazaki S, Toyota T, Ohara K, Ohtaka M, Nakanishi M, Shin C, Ota Y, Nakashima K, Ohshima K, Imai C, Nakazawa Y, Nakauchi H, Komatsu N.
Dual-antigen targeted iPSC-derived chimeric antigen receptor-T cell therapy for refractory lymphoma.
Mol Ther. 2022 Feb

Abstract on PubMed

Furukawa Y, Hamano Y, Shirane S, Kinoshita S, Azusawa Y, Ando J, Nakauchi H, Ando M.
Advances in Allogeneic Cancer Cell Therapy and Future Perspectives on "Off-the-Shelf" T Cell Therapy Using iPSC Technology and Gene Editing.
Cells. 2022 Jan

Abstract on PubMed

Watanabe M, Umeyama K, Nakano K, Matsunari H, Fukuda T, Matsumoto K, Tajiri S, Yamanaka S, Hasegawa K, Okamoto K, Uchikura A, Takayanagi S, Nagaya M, Yokoo T, Nakauchi H, Nagashima H.
Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation.
Lab Invest. 2022 Jan

Abstract on PubMed

Kinoshita M, Kobayashi T, Planells B, Klisch D, Spindlow D, Masaki H, Bornelöv S, Stirparo GG, Matsunari H, Uchikura A, Lamas-Toranzo I, Nichols J, Nakauchi H, Nagashima H, Alberio R, Smith A.
Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.
Development. 2021 Dec

Abstract on PubMed

Kobayashi T, Castillo-Venzor A, Penfold CA, Morgan M, Mizuno N, Tang WWC, Osada Y, Hirao M, Yoshida F, Sato H, Nakauchi H, Hirabayashi M, Surani MA.
Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells.
Cell Rep. 2021 Oct

Abstract on PubMed

Ishii M, Ando J, Yamazaki S, Toyota T, Ohara K, Furukawa Y, Suehara Y, Nakanishi M, Nakashima K, Ohshima K, Nakauchi H, Ando M.
iPSC-Derived Neoantigen-Specific CTL Therapy for Ewing Sarcoma.
Cancer Immunol Res. 2021 Oct

Abstract on PubMed

Harada S, Ando M, Ando J, Ishii M, Yamaguchi T, Yamazaki S, Toyota T, Ohara K, Ohtaka M, Nakanishi M, Shin C, Ota Y, Nakashima K, Ohshima K, Imai C, Nakazawa Y, Nakauchi H, Komatsu N
Dual-antigen targeted iPSC-derived chimeric antigen receptor-T cell therapy for refractory lymphoma.
Mol Ther. 2021 Oct

Abstract on PubMed

Sudo K, Yamazaki S, Wilkinson AC, Nakauchi H, Nakamura Y.
Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.
Stem Cell Res. 2021 Oct

Abstract on PubMed

Bouchareychas L, Duong P, Phu TA, Alsop E, Meechoovet B, Reiman R, Ng M, Yamamoto R, Nakauchi H, Gasper WJ, Van Keuren-Jensen K, Raffai RL.
High glucose macrophage exosomes enhance atherosclerosis by driving cellular proliferation & hematopoiesis.
iScience. 2021 Jul

Abstract on PubMed

Nagaya M, Hasegawa K, Uchikura A, Nakano K, Watanabe M, Umeyama K, Matsunari H, Osafune K, Kobayashi E, Nakauchi H, Nagashima H.
Feasibility of large experimental animal models in testing novel therapeutic strategies for diabetes.
World J Diabetes. 2021 Apr

Abstract on PubMed

Kobayashi T, Goto T, Oikawa M, Sanbo M, Yoshida F, Terada R, Niizeki N, Kajitani N, Kazuki K, Kazuki Y, Hochi S, Nakauchi H, Surani MA, Hirabayashi M.
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
Nat Commun. 2021 Feb

Abstract on PubMed

Wilkinson AC, Dever DP, Baik R, Camarena J, Hsu I, Charlesworth CT, Morita C, Nakauchi H, Porteus MH.
Cas9-AAV6 gene correction of beta-globin in autologous HSCs improves sickle cell disease erythropoiesis in mice.
Nat Commun. 2021 Jan

Abstract on PubMed

Nishimura T, Suchy FP, Bhadury J, Igarashi KJ, Charlesworth CT, Nakauchi H.
Generation of Functional Organs Using a Cell-Competitive Niche in Intra-and Inter-species Rodent Chimeras.
Cell Stem Cell. 2021 Jan

Abstract on PubMed

Nagaya M, Hasegawa K, Watanabe M, Nakano K, Okamoto K, Yamada T, Uchikura A, Osafune K, Yokota H, Nagaoka T, Matsunari H, Umeyama K, Kobayashi E, Nakauchi H, Nagashima H.
Genetically engineered pigs manifesting pancreatic agenesis with severe diabetes.
BMJ Open Diabetes Res Care. 2020 Nov

Abstract on PubMed

Yamamoto R, Nakauchi H.
In vivo clonal analysis of aging hematopoietic stem cells.
Mech Ageing Dev. 2020 Oct

Abstract on PubMed

Wilkinson AC, Igarashi KJ, Nakauchi H.
Haematopoietic stem cell self-renewal in vivo and ex vivo.
Nat Rev Genet. 2020 Sep

Abstract on PubMed

Honda T, Ando M, Ando J, Ishii M, Sakiyama Y, Ohara K, Toyota T, Ohtaka M, Masuda A, Terao Y, Nakanishi M, Nakauchi H, Komatsu N.
Sustainable Tumor-Suppressive Effect of iPSC-Derived Rejuvenated T Cells Targeting Cervical Cancers.
Mol Ther. 2020 Jul

Abstract on PubMed

Nishimura T, Xu H, Iwasaki M, Karigane D, Saavedra B, Takahashi Y, Suchy FP, Monobe S, Martin RM, Ohtaka M, Nakanishi M, Burrows SR, Cleary ML, Majeti R, Shibuya A, Nakauchi H.
Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells.
Gene Ther. 2020 Jul

Abstract on PubMed

Bouchareychas L, Duong P, Covarrubias S, Alsop E, Phu TA, Chung A, Gomes M, Wong D, Meechoovet B, Capili A, Yamamoto R, Nakauchi H, McManus MT, Carpenter S, Van Keuren-Jensen K, Raffai RL.
Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo.
Cell Rep. 2020 Jul

Abstract on PubMed

Onodera S, Saito A, Hojo H, Nakamura T, Zujur D, Watanabe K, Morita N, Hasegawa D, Masaki H,Nakauchi H, Nomura T, Shibahara T, Yamaguchi A, Chung UI, Azuma T, Ohba S.
Hedgehog Activation Regulates Human Osteoblastogenesis.
Stem Cell Reports. 2020 Jul

Abstract on PubMed

Yamamoto R, Wilkinson AC, Nakauchi H.
In vivo and ex vivo haematopoietic stem cell expansion.
Curr Opin Hematol. 2020 Jul

Abstract on PubMed

Wilkinson AC, Nakauchi H.
Stabilizing hematopoietic stem cells in vitro.
Curr Opin Genet Dev. 2020 Jun

Abstract on PubMed

Wilkinson AC, Igarashi KJ, Nakauchi H.
Haematopoietic stem cell self-renewal in vivo and ex vivo.
Nat Rev Genet. 2020 May

Abstract on PubMed

Sato A, Kakinuma S, Miyoshi M, Kamiya A, Tsunoda T, Kaneko S, Tsuchiya J, Shimizu T, Takeichi E, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Koshikawa N, Seiki M, Nakauchi H, Asahina Y, Watanabe M.
Vasoactive Intestinal Peptide Derived From Liver Mesenchymal Cells Mediates Tight Junction Assembly in Mouse Intrahepatic Bile Ducts.
Hepatol Commun. 2020 Feb

Abstract on PubMed

Kobayashi T, Kobayashi H, Goto T, Takashima T, Oikawa M, Ikeda H, Terada R, Yoshida F, Sanbo M, Nakauchi H, Kurimoto K, Hirabayashi M.
Germline development in rat revealed by visualization and deletion of Prdm14.
Development. 2020 Jan

Abstract on PubMed

Zujur D, Kanke K, Onodera S, Tani S, Lai J, Azuma T, Xin X, Lichtler AC, Rowe DW, Saito T, Tanaka S, Masaki H, Nakauchi H, Chung UI, Hojo H, Ohba S.
Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.
Regen Ther. 2020 Jan

Abstract on PubMed

Wilkinson AC, Ishida R, Nakauchi H, Yamazaki S.
Long-term ex vivo expansion of mouse hematopoietic stem cells.
Nat Protoc. 2020 Jan

Abstract on PubMed

Matsunari H, Watanabe M, Hasegawa K, Uchikura A, Nakano K, Umeyama K, Masaki H, Hamanaka S, Yamaguchi T, Nagaya M, Nishinakamura R, Nakauchi H, Nagashima H.
Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation.
Stem Cell Reports. 2020 Jan

Abstract on PubMed