Publications

Paper & Review

Adhesion patterning by a novel air-lock technique enables localization and in-situ real-time imaging of reprogramming events in one-to-one electrofused hybrids.
Sakamoto S, Okeyo KO, Yamazaki S, Kurosawa O, Oana H, Kotera H, Washizu M.
Biomicrofluidics. 2016 Oct 27;10(5):054122

Abstract on PubMed

Yuki Taya, Yasunori Ota, Adam C Wilkinson, Ayano Kanazawa, Hiroshi Watarai, Masataka Kasai, Hiromitsu Nakauchi and Satoshi Yamazaki.
Depleting dietary valine permits nonmyeloablative mouse hematopoietic stem cell transplantation. Science. Oct 20, 2016

Pre-transplantation blockade of TNF-α-mediated oxygen species accumulation protects hematopoietic stem cells.
Ishida T, Suzuki S, Lai CY, Yamazaki S, Kakuta S, Iwakura Y, Nojima M, Takeuchi Y, Higashihara M, Nakauchi H, Otsu M.
Stem Cells. 2016 Oct 18. doi: 10.1002/stem.2524.

Abstract on PubMed

Koide S, Oshima M, Takubo K, Yamazaki S, Nitta E, Saraya A, Aoyama K, Kato Y, Miyagi S, Nakajima-takagi Y, Chiba T, Matsui H, Arai F, Suzuki Y, Kimura H, Nakauchi H, Suda T, Shinkai Y, Iwama A. Setdb1 maintains hematopoietic stem and progenitor cells by restricting the ectopic activation of non-hematopoietic genes. Blood. Jun 14 , 2016

Abstract on PubMed

Chen JY, Miyanishi M, Wang SK, Yamazaki S, Shnha R, Kao KS, Seita J, Sahoo D, Nakauchi H, Weissman IL. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenyous perivascular niche. Nature. 530(7589):223-7, 2016.

Abstract on PubMed

Ando M, Nishimura T, Yamazaki S, Yamaguchi T, Kawana-Tachikawa A, Hayama T, Nakauchi Y, Ando J, Ota Y, Takahashi S, Nishimura K, Ohtaka M, Nakanishi M, Miles JJ, Burrows SR, Brenner MK, Nakauchi H. A Safeguard System for Induced Pluripotent Stem Cell Derived Rejuvenated T Cell Therapy. Stem Cell Reports. 5(4): 597-608, 2015.

Abstract on PubMed

Ishida T, Yamazaki S, Nakauchi H, Higashihara M, Otsu M. Reactive oxygen species in hematopoietic stem cells affect culture outcome under inflammatory conditions. Open Journal of Hematology. 6: 6-7, 2015.

Okeyo KO, Kurosawa O, Yamazaki S, Oana H, Kotera H, Nakauchi H, Washizu M. Cell Adhesion Minimization by a Novel Mesh Culture Method Mechanically Directs Trophoblast Differentiation and Self-Assembly Organization of Human Pluripotent Stem Cells. Tissue Eng Part C Methods. 21(10):1105-15, 2015.

Nakauchi Y, Yamazaki S*, Napier C S, Usui J, Ota Y, Takahashi S, Watanabe N, Nakauchi H. (*Correspondence author) Effective treatment against severe Graft-versus-Host Disease with allele-specific anti-HLA monoclonal antibody in a humanized-mouse model. Exp Hematol. 43(2):79-88, 2015.

Ito T, Sendai Y, Yamazaki S, Seki-Soma M, Hirose K, Watanabe M, Fukawa K, Nakauchi H. Generation of recombination activating gene-1-deficient neonatal piglets: a model of T and B cell deficient severe combined immune deficiency. PLoS One. 9(12):e113833, 2014.

Hayama T, Yamaguchi T, Kato-Itoh M, Hamanaka S, Kawarai M, Sanbo M, Tamura C, Lee YS, Yanagida A, Murayama H, Mizuno N, Umino A, Sato H, Yamazaki S, Masaki H, Kobayashi T, Hirabayashi M, Nakauchi H. Generation of Mouse Functional Oocytes in Rat by Xeno-Ectopic Transplantation of Primorial Germ Cells. Biol Reprod. 91(4):89, 2014.

Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimisu R, Nakauchi H, Yamamoto T, Oshimura M. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies. Sci Rep. 4: 6136, 2014.

Ito K, Yamazaki S, Yamamoto R, Tajima Y, Yanagida A, Kobayashi T, Kato-Itoh M, Kakuta S, Iwakura Y, Nakauchi H, Kamiya A. Gene Targeting Study Reveals Unexpected Expression of Brain Expressed X-linked 2 in Endocrine and Tissue Stem/projenitor Cells in Mice. J Biol Chem. 289(43):29892-911, 2014.

Okamoto N, Aoto T, Uhara H, Yamazaki S, Akutsu H, Umezawa A, Nakauchi H, Miyachi Y, Saida T, Nishimura EK. A melanocyte-melanoma precursor niche in sweat glands of volar skin. Pigment Cell Melanoma. 27(6):1039-50, 2014.

Sakurai M, Kunimoto H, Watanabe N, Fukuchi Y, Yuasa S, Yamazaki S, Nishimura T, Sadahira K, Fukuda K, Okano H, Nakauchi H, Morita Y, Matsumura I, Kudo K, Ito E, Ebihara Y, Tsuji K, Harada Y, Harada H, Okamoto S, Nakajima H. Impaired hematopoietic differentiation of RUNX1 mutated induced pluripotent stem cells derived from FPD/AML patients. Leukemia. 28(12):2344-54, 2014.

Ieyasu A, Tajima Y, Shimba S, Nakauchi H, Yamazaki S*. (*Correspondence author) Clock gene Bmal1 is dispensable for intrinsic properties of murine hematopoietic stem cells. J Negat Biomed. 13: 4, 2014.

Miyagi S, Koide S, Saraya A, Wendt GR, Oshima M, Konuma T, Yamazaki S, Mochizuki-Kashio M, Nakajima-Takagi Y, Wang C, Chiba T, Kitabayashi I, Nakauchi H, Iwama A. The TIF1beta-HP1 System Maintains Transcriptional Integrity of Hematopoietic Stem Cells. Stem Cell Reports. 2(2):145-52, 2014.

Lai CY, Yamazaki S, Okabe M, Suzuki S, Maeyama Y, Iimura Y, Onodera M, Kakuta S, Iwakura Y, Nojima M, Otsu M, Nakauchi H. Stage-specific roles for Cxcr4 signaling in murine hematopoietic stem/progenitor cells in the process of bone marrow repopulation. Stem cells. 32(7):1929-42, 2014.

Hirose S, Takayama N, Nakamura S, Nagasawa K, Ochi K, Hirata S, Yamazaki S, Yamaguchi T, Otsu M, Sano S, Takahashi N, Sawaguchi A, Ito M, Kato T, Nakauchi H, Eto K. Immortalization of erythroblast by c-Myc and Bcl-xl enables large-scale erythrocyte production from human pluripotent stem cells. Stem Cell Reports. 1(6):499-508, 2013.

Yamazaki S*, Suzuki N, Yamaguchi T, Okabe M, Masaki H, Takaki S, Otsu M and Nakauchi H. (*Correspondence author) Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation. Mol Ther. 21(7): 1424-31, 2013.

Nakamura S, Oshima M, Yuan J, Saraya A, Miyagi S, Konuma T, Yamazaki S, Osawa M, Nakauchi H, Koseki H and Iwama A. Bmi-1 confers resistance to oxidative stress on hematopoietic stem cells. PLoS One. 7(5):e36209, 2012.

Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Kobayashi T, Yamazaki S and Nakauchi H.
Development of an all in one inducible lentiviral vector for gene specific analysis of reprogramming. PLoS One. 7(7): e41007, 2012.

Oguro H, Yuan J, Tanaka S, Miyagi S, Mochizuki-Kashio M, Ichikawa H, Yamazaki S, Koseki H, Nakauchi H and Iwama A. Lethal myelofibrosis induced by Bmi-1 deficient hematopoietic stem cells unveils a tumor suppressor function of the polycomb group genes. J Exp Med. 209(3):445-454, 2012.

Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, and Nakauchi H. Non-myelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Cell. 23, 1146-1158, 2011.

Suzuki N, Yamazaki S, Ema H, Yamaguchi T, Nakauchi H, and Nakauchi H. Homeostasis of hematopoietic stem cells regulated by the myeloproliferative disease associated-gene product Lnk/Sh2b3 via Bcl-xl. Exp Hematol. 40, 166-174, 2011.

Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H. Generation of germline-competent rat induced pluripotent stem cells. PLoS One. 6(7):e22008, 2011.

Tanimura S, Tadokoro Y, Inomata K, Binh NT, Nishie W, Yamazaki S, Nakauchi H, Tanaka Y, McMillan JR, Sawamura D, Yancey K, Shimizu H, Nishimura EK. Hair follicle stem cells provide a functional niche for melanocyte stem cells. Cell Stem Cell. 8(2):177-87, 2011.

Nakahata S, Yamazaki S, Nakauchi H, Morishita K. Downregulation of ZEB1 and overexpression of Smad7 contribute to resistance to TGF-beta1-mediated growth suppression in adult T-cell leukemia/lymphoma. Oncogene. 29(29):4157-69, 2010.

Yamanishi Y, Kitaura J, Izawa K, Kaitani A, Komeno Y, Nakamura M, Yamazaki S, Enomoto Y, Oki T, Akiba H, Abe T, Komori T, Morikawa Y, Kiyonari H, Takai T, Okumura K, Kitamura T. TIM1 is an endogenous ligand for LMIR5/CD300b: LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury. J Exp Med. 207(7): 1501-11, 2010.

Oguro H, Yuan J, Ichikawa H, Ikawa T, Yamazaki S, Kawamoto H, Nakauchi H, Iwama A. Poised lineage specification in multipotential hematopoietic stem and progenitor cells by the polycomb protein Bmi1. Cell Stem Cell. 6(3):279-86, 2010.

Takizawa H, Nishimura S, Takayama N, Oda A, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, Nakauchi H, Takaki S, Eto K. Lnk regulates integrin alphaIIbbeta3 outside-in signaling in mouse platelets, leading to stabilization of thrombus development in vivo. J Clin Invest. 120(1):179-90, 2009.

Sanada M, Suzuki T, Shih LY, Otsu M, Kato M, Yamazaki S, Tamura A, Honda H, Sakata-Yanagimoto M, Kumano K, Oda H, Yamagata T, Takita J, Gotoh N, Nakazaki K, Kawamata N, Onodera M, Nobuyoshi M, Hayashi Y, Harada H, Kurokawa M, Chiba S, Mori H, Ozawa K, Omine M, Hirai H, Nakauchi H, Koeffler HP, Ogawa S. Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms. Nature. 460(7257):904-8, 2009.

Yamazaki S, Suzuki N, Saito T, Ishii Y, Takiguchi M, Nakauchi H, Watanabe N. A rapid and efficient strategy to generate allele-specific anti-HLA monoclonal antibodies. J Immunol Methods. 343(1):56-60, 2009.

Yamazaki S, Iwama A, Takayanagi S, Eto K, Ema H, Nakauchi H. TGF-beta as a candidate bone marrow niche signal to induce hematopoietic stem cell hibernation. Blood. 113(6):1250-6, 2008.

Yamanishi Y, Kitaura J, Izawa K, Matsuoka T, Oki T, Lu Y, Shibata F, Yamazaki S, Kumagai H, Nakajima H, Maeda-Yamamoto M, Tybulewicz VL, Takai T, Kitamura T. Analysis of mouse LMIR5/CLM-7 as an activating receptor: differential regulation of LMIR5/CLM-7 in mouse versus human cells. Blood. 111(2):688-98, 2008.

Miyamoto K, Araki KY, Naka K, Arai F, Takubo K, Yamazaki S, Matsuoka S, Miyamoto T, Ito K, Ohmura M, Chen C, Hosokawa K, Nakauchi H, Nakayama K, Nakayama KI, Harada M, Motoyama N, Suda T, Hirao A. Foxo3a is essential for maintenance of the hematopoietic stem cell pool. Cell Stem Cell. 1(1):101-12, 2007.

Ema H, Morita Y, Yamazaki S, Matsubara A, Seita J, Tadokoro Y, Kondo H, Takano H, Nakauchi H. Adult mouse hematopoietic stem cells: purification and single-cell assays. Nat Protoc. 1(6): 2979-87, 2006.

Seita J, Ema H, Ooehara J, Yamazaki S, Tadokoro Y, Yamasaki A, Eto K, Takaki S, Takatsu K, Nakauchi H. Lnk negatively regulates self-renewal of hematopoietic stem cells by modifying thrombopoietin-mediated signal transduction. Proc Natl Acad Sci U S A. 104(7): 2349-54, 2007.

Yamazaki S, Iwama A, Takayanagi S, Morita Y, Eto K, Ema H, Nakauchi H. Cytokine signals modulated via lipid rafts mimic niche signals and induce hibernation in hematopoietic stem cells. EMBO J. 25(15):3515-23, 2006.

Morita Y, Ema H, Yamazaki S, Nakauchi H. Non-side-population hematopoietic stem cells in mouse bone marrow. Blood. 108(8):2850-6, 2006.

Takayanagi S, Hiroyama T, Yamazaki S, Nakajima T, Morita Y, Usui J, Eto K, Motohashi T, Shiomi K, Keino-Masu K, Masu M, Oike Y, Mori S, Yoshida N, Iwama A, Nakauchi H. Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain. Blood. 107(11):4317-25, 2006.

Usui J, Osawa M, Yamazaki S, Morita Y, Koyama A, Nakauchi H. Identification of immature podocyte specific antigen using retrovirus-mediated gene transfer and cell sorting. Clin Exp Nephrol. 9(4):292-6, 2005.

Matsubara A, Iwama A, Yamazaki S, Furuta C, Hirasawa R, Morita Y, Osawa M, Motohashi T, Eto K, Ema H, Kitamura T, Vestweber D, Nakauchi H. Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development. J Exp Med. 202(11):483-92, 2005.

Tahara-Hanaoka S, Shibuya K, Onoda Y, Zhang H, Yamazaki S, Miyamoto A, Honda S, Lanier LL, Shibuya A. Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112). Int Immunol. 16(4):533-8, 2004.

Yotsumoto K, Okoshi Y, Shibuya K, Yamazaki S, Tahara-Hanaoka S, Honda S, Osawa M, Kuroiwa A, Matsuda Y, Tenen DG, Iwama A, Nakauchi H, Shibuya A. Paired activating and inhibitory immunoglobulin-like receptors, MAIR-I and MAIR-II, regulate mast cell and macrophage activation. J Exp Med. 198(2):223-33, 2003.

Conference Presentation

K. Izawa, T. Kakegawa, M. Yamamoto, A. Tojo.
HOXB4 sustains iPSC-derived CD45- pre-HSC which develop LT- or ST- HS/PCs according to GATA2 level. The Japanese Society of Hematology in Kanazawa, 2015

Activity in the lab