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Mast cells are considered to be involved in the development of allergic
disease and inflammation. However, it was also indicated that the activation
of mast cells in the early time point of infection seemed to be important
for the development of effective immune responses against pathogenic bacteria.
These evidences imply that adequate activation of mast cells is required
for the protection of our body from invasive pathogenic organism but inadequate
activation caused inflammatory disorders. To figure out the regulatory
mechanisms of mast cell activation in both effective protective immunity
and inflammation-associated infection，the use of infection-associated inflammation
mouse models seem to be adequate for analysis.
Recently, innate immune responses to Chlamydia pneumoniae infection
and Kawasaki-disease have been actively analyzed by the group of Professor
Moshe Arditi (Crother TR et. al., PLoS One. 2012, Lee Y. et. al., Circulation.
2012, Shimada K. et. al., Immunity. 2012). During the visit, I examined
the possible roles of mast cells in the infection-associated inflammation,
such as Chlamydia pneumonia-mediated inflammatory disorders. We also aimed
to evaluate the machinery of mast cell-activation in this infection-associated
inflammation with our newly established monoclonal antibodies. Since this
program for dispatch, we have started evaluation of the involvement of
mast cells in the protection or inflammation in the disease above, especially
based on our novel founding which demonstrated the importance of mast cells
in the innate type of immune responses in mucosal compartments (Kurashima
Y. et al., Nat Commun 2012). I learned the experimental procedure of Chlamydia
pneumoniae infection and Kawasaki disease in this period. In addition,
it was great and precious opportunity for me to come in touch with collaborative
and unique styles of foreign laboratories.
 
Experiment now With Boss |
