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  1. Identification of new biomarkers and molecular targets of lung cancers by systems biology approach 1. Identification of new biomarkers and molecular targets of lung cancers by systems biology approach
  Molecular mechanisms of cancer initiation, progression, invasion and metastasis: establishment of breast cancer model 2. Molecular mechanisms of cancer initiation, progression, invasion and metastasis: establishment of breast cancer model
  Signal transduction mechanisms through RTKs for tumorigenesis, stem cell maintenance, and development 3. Signal transduction mechanisms through RTKs for tumorigenesis, stem cell maintenance, and development

2. Molecular mechanisms of cancer initiation, progression, invasion and metastasis: establishment of breast cancer model

The hypothesis of cancer stem cells is that a small population in cancer tissues has ability to self-renew, serving as a reservoir for all the rest of the cancer cells. To clarify the mechanisms how small numbers of cancer stem cells expand and make a large tumor, we attempted to establish a mouse model using breast cancer cell lines. We introduced luciferase reporter into several breast cancer cell lines by lentivirus and isolated the CD24-/low CD44+ population, that are believed to be enriched with cancer stem cells, and the control population (CD24+ CD44+). We implanted CD24-/low CD44+ and control population to the mammary fat pads of NOD-SCID mice and examined tumor growth by in vivo imaging using the luciferase reporter. Using this model, we are currently analyzing the complex time series molecular mechanisms of tumorigenesis arisen from cancer stem cells.


Figure 2. Potential inflammatory signaling pathways in breast cancer stem cells.
Breast cancer stem cells may be regulated by chemokine- and/or cytokine-mediated inflammatory signaling in an autocrine or paracrine manner.

  1. Identification of new biomarkers and molecular targets of lung cancers by systems biology approach 1. Identification of new biomarkers and molecular targets of lung cancers by systems biology approach
  Molecular mechanisms of cancer initiation, progression, invasion and metastasis: establishment of breast cancer model 2. Molecular mechanisms of cancer initiation, progression, invasion and metastasis: establishment of breast cancer model
  Signal transduction mechanisms through RTKs for tumorigenesis, stem cell maintenance, and development 3. Signal transduction mechanisms through RTKs for tumorigenesis, stem cell maintenance, and development

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University of TokyoInstitute of Medical Science
Division of Molecular Therapy, Molecular Targets Laboratory
(former Division of Systems Biomedical Technology)
 Institute of Medical Science, University of Tokyo  
4-6-1 Shirokanedai, Minato-ku Tokyo, Japan, 108-8639 TEL:+81-3-5449-5629