Non-small cell lung cancer (NSCLC) is the most common histological subtype
of lung cancer. Because the adjuvant chemotherapy for the patients with
the early stage of lung cancer after surgery causes the treatment-related
toxicity, the patients undergo only surgery. However about 20% of NSCLC
patients diagnosed as stage I underwent surgery, they eventually died due
to recurrence. Therefore, it is very important to classify patients with
early stage lung adenocarcinoma into groups with low-risk or high-risk
of recurrence. We have already identified the EGF-influenced 139 genes
as the candidates to predict the survival of patients with early stage
lung adenocarcinoma (PLoS ONE, vol. 7, e43923, 2012). To develop a clinically-available
diagnostic system, it is necessary to examine the expression levels of
the genes using quantitative RT-PCR (qRT-PCR) on a large number of patients
tissues and to construct a risk scoring model by optimizing for qRT-PCR
to predict risk.
Dr. David G Beer has collected a large number of samples from patients
with NSCLC for long time and has already reported several excellent papers
to analyze lung adenocarcinoma patients’ samples. I visited his laboratory
at the University of Michigan Medical School to collect the data using
their precious patient’s samples and to learn the methods for the construction
of a risk-scoring model based on qRT-PCR. During the stay, I could measure
totally 216 samples by qRT-PCR and identified some genes that correlated
with NSCLC patients’ survival. I have been constructing a risk-scoring
model based on these qRT-PCR data. Furthermore, I could also obtain the
data of miRNA expression from patient’s serum. We’re also analyzing the
correlation between miRNA expression and patients’ survival.
I could also enjoy many interesting seminars. On every Thursday, we attended
the seminar with lunch provided. This lunch seminars were planned as a
part of education for students and young researchers. Many busy physicians
and some PIs also attended the seminar and got the chance to new collaboration.
I looked forward to the seminar with bagel and coffee provided on every
Friday morning, called “Bagel Talk”, to have a chance to discuss with other
laboratory members. I really appreciate having this precious experience
and would like to strongly recommend other student and young researcher
to have the experience abroad.
Wtih Boss Lab Members
During my experimet Before "Bagel Talk"