| The protein of MTHFD2 is located in mitochondria matrix, and functions
for one-carbon metabolism. To examine the metabolic changes with the knockdown
of MTHFD2, it is necessary to quantify the intracellular metabolite concentrations
using mass spectrometery.
I went to the Lysosomal Diseases Research Unit, the Women’s and Children’s
Hospital, in Adelaide, South Australia. In this Dr. John’s lab, there are
mass spec staffs who developed the methods to measure various molecules.
They do analysis in lots of different kinds of samples (e.g., fibroblast
cell cultures, tissue homogenates). I learned some general mass spec methods
from them. How to adjust the mass spec machine for each sample, and what
is read out from the data. I had many chances to discuss with them, so
it was very meaningful time for me. Now I’m planning to examine the changes
of metabolites with the knockdown of MTHFD2 by mass spec. I expect that
this project will contribute to find out the molecular mechanisms; how
MTHFD2 functions in cancer cells.
I appreciate the fund from Japan Society for the Promotion of Science (JSPS)
for the “Institutional Program for Young Researcher Overseas Visits” and
its management staffs for this great experience.
Lab Members At Lunch Time