The mechanism of coronavirus RNA packaging

学友会セミナー

2002年開催　学友会セミナー

開催日時：	2002年6月13日（木）11:00 ～ 12:00
開催場所：	東京大学医科学研究所　アムジェンホール大会議室
講　　師：	Dr. Shinji Makino
所　　属：	Professor, Department of Microbiology and Immunology, The University of Texas Medical Branch
演　　題：	The mechanism of coronavirus RNA packaging
概　　要：	For any of the enveloped RNA viruses studied to date, recognition of a specific RNA packaging signal (PS) by the virus's nucleocapsid (N) protein is the first step described in the process of viral RNA packaging. I will show that expressed coronavirus transmembrane envelope protein M specifically interacted with coexpressed non-coronavirus RNA transcripts containing the short viral PS in the absence of coronaviurs N protein. Furthermore, this M protein-PS interaction led to specific packaging of the PS-containing RNA transcripts into coronavirus-like particles in the absence of N protein. These finding not only highlight a novel RNA packaging mechanism for an enveloped virus, where the specific RNA packaging can occur without the core or N protein, but also point to a new biologically important general model of precise and selective interaction between transmembrane proteins and specific RNA elements.
世 話 人：	河岡　義裕 甲斐知恵子