OVA-Protein Sigma 1 M Cell Targeting Enhances Oral Tolerance With Loss Of OVA-Specific CD4+ T Cells
学友会セミナー
2005年開催 学友会セミナー
開催日時: | 平成17年8月8日(月) 16:00~17:30 |
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開催場所: | 総合研究棟4F会議室 |
講 師: | Dr. Kotaro Fujihashi |
所 属: | Professor of Pathology and Microbiology, University of Alabama at Birmingham |
演 題: | "OVA-Protein Sigma 1 M Cell Targeting Enhances Oral Tolerance With Loss Of OVA-Specific CD4+ T Cells" |
概 要: | We examined whether M cell targeting using a protein Ag delivery system would facilitate induction of oral tolerance. We have developed a recombinant protein sigma one of reovirus genetically conjugated with ovalbumin(OVA-pσ1). Mice were fed different doses of OVA-pσ1or PBS prior to oral challenge with OVA plus cholera toxin as mucosal adjuvant. Feeding a low dose of OVA-pσ1 resulted in reduced anti-OVA antibody and CD4-positive(CD4+ ) T cell responses in both mucosal and systemic lymphoid tisseues. OVA / MHC I-Adtetramer staining revealed a significant reduction in the numbers of OVA-specific CD4+ T cells in the intestinal lamina propria but not spleen of mice fed pσ1-OVA when compared with those fed PBS. An increased frequency of CD25+, CD4+ T cells were present in the spleen of orally tolerized mice.These results show that mucosal and systemic unresponsiveness are regulated by distinct T cell subsets. |
世 話 人: | ○炎症免疫分野 清野 宏 ウィルス感染分野 河岡 義裕 |