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Nuclear receptor CAR and adaptive expression of genes in response to drugs

学友会セミナー

学友会セミナー

2002年開催 学友会セミナー

開催日時: 2002年4月1日(月)16:00 ~ 17:00
開催場所: 東京大学医科学研究所 クレストホール会議室
講  師: Dr. Masahiko Negishi
所  属: Head, Pharmacogenetics section
Laboratory of Reproductive and Developmental Toxicology, National Institutes of Health, USA
演  題: Nuclear receptor CAR and adaptive expression of genes in response to drugs
概  要:

核内レセプターCARについて先端的に研究を進めており、今回は以下のような話題を提供していただきます。


”Phenobarbital (PB) induces various gene encoding drug/steroid-metabolizing enzymes such as cytochromes P450 (P450s) and transferases. Although the nuclear orphan constitutive active receptor (CAR) has been identified as a key transcription factor that regulates the induction of CYP2B, the full scope of CAR-regulated genes still remains a major question. To this end, RT-PCR and cDNA microarray techniques were employed to examine gene expression in wild-type and CAR-null mice. The results show that a total of 138 genes were detected to be either induced or repressed in response to PB treatment, of which about half were under CAR regulation. Analysis of the expression and function of these genes suggested that CAR plays diverse roles, both as a positive and negative regulator, in the regulation of hepatic genes in response to PB beyond drug/steroid metabolism.

世 話 人: 山本 雅
伊庭 英夫