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Catalytic RNA meets selfish DNA: group I introns and their homing endonucleases.

学友会セミナー

学友会セミナー

2004年開催 学友会セミナー

開催日時: 平成16年4月16日(金)16:00~17:00
開催場所: 1号館2階会議室
講  師: Dr. David Shub
所  属: アルバニー大学 教授
演  題: Catalytic RNA meets selfish DNA: group I introns and their homing endonucleases.
概  要:

Self-splicing group I introns have invaded ribosomal RNA genes of eukaryotes, and a wide variety of genes of mitochondria, chloroplasts, bacteria and bacteriophages. The spread of these introns has been facilitated by "homing" endonucleases, encoded by genes that are inserted into the introns. The endonucleases recognize and cleave the fused exon sequences of cognate genes that lack an intron, promoting the spread of the intron by replicative repair of the cleaved DNA. Dr. Shub will present studies of the relationship between introns and their homing endonucleases. Genetic experiments with bacteriophages reveal that genes for homing endonucleases are themselves selfish elements, capable of spread through populations independently of their association with an intron. Recent investigations of phage introns give insight into how a self-catalytic splicing element can acquire an endonuclease that is already pre-adapted to cleave the vacant (intronless) site of cognate genes.

「シュブ博士はイントロンの起源、進化、機能とエクソンシャフリングの解析で画期的な成果をあげてきました。『イントロンによって分断されているゲノム配列を切断する特異性を既に持つ酵素が、イントロンに侵入することによって、動くイントロンが成立した』という仮説の証拠について話して頂きます。」

世 話 人: ○中村 義一、中井 謙太、井ノ上 逸朗