東京大学医科学研究所

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学友会セミナー

学友会セミナー:2019年8月23日

開催日時: 2019年8月23日 14:00 ~ 15:00
開催場所: 2号館 小講義室
講師: Constance Alabert
所属: University of Dundee, Center of Gene Regulation and Expression
Principal Investigator
演題: Restoration of chromatin based information behind replication forks
概要:

Epigenetic states defined by chromatin must be maintained through mitotic division during cell propagation. Using Nascent Chromatin Capture coupled to quantitative mass spectrometry we showed that post-translational modifications (PTMs) are diluted twofold upon DNA replication, as a consequence of new histone deposition (Alabert et al. 2015). We could observe that for most of PTMs, the pre-replication state is restored by new histones being modified to mirror the parental histones. However, H3K9 trimethylation (H3K9me3) and H3K27me3 are propagated by continuous modification of parental and new histones (Alabert et al. 2015). Here we report that parental histones are important to promote PTM restoration on new histones. Our data further reveal that newly synthesized histones and recycled parental histones are not processed by similar mechanisms. Consequently, restoration of PTMs on new histones must be executed on time upon their assembly in order to maintain PTMs level in cycling cells. Together, our results highlight the importance of the PTM restoration program in cycling cells.

世話人: 〇中西 真 (癌防御シグナル分野)
 岩間 厚志(幹細胞分子医学分野)